Cryopreservation Techniques in Stem Cell Banking

Cryopreservation Techniques in Stem Cell Banking

Did you know that it is possible to preserve your stem cells for future use in the future by cryopreservation? Currently, this is the only possible method whereby Cryopreservation in stem cell banking is the process of ensuring that the cells are stored for a very long time at very low temperatures with the hope that they will be applicable in a wide range of fields. The practice seems to have a number of challenges; however, cutting off and freezing your cells does seem to be worth it, in addition to having the equipment and reagents in place. Let us take a look at the process of cryopreservation in banked stem cells in detail and how it works!

Cryopreservation and Its Purpose in Stem Cell Banking

Cryopreservation refers to the technique of preserving cells and tissues at extremely low templates to ensure their extended storage and viability. By carefully freezing cells and rejuvenating the formation of crystals, the technique ensures that the cells can be stored for a long period without compromising their integrity. This is how cryopreservation has found its way into clinical applications, especially in stem cell banking. 

Cryopreservation Steps - An Overview

The cryopreservation technique supports cutting-edge research and facilitates breakthroughs in various scientific disciplines,  allowing the establishment of different cell banks. Here are the complete procedure steps involved in preserving the obtained cord blood are as follows:
  1. Cell preparation - Before cryopreservation, stem cells have to be carefully prepared to withstand freezing. To achieve consistent cell accounts, the filling should be accompanied by the homogenization of cells. 
  2. Addition of cryo-protectant - Cryoprotective agents such as glycerol, salts, sugars, and glycols are added to the samples. It will rescue the freezing point to the medium and also allow a slower cooling rate, reducing the risk of crystallization.
  3. Freezing - Different methods of freezing are applied in the cryopreservation technique to protect stem cells from damage. It also prevents cell death by their exposure to the warm solutions of cryoprotective agents.
  4. Storage in Liquid Nitrogen - The cryopreserved stem cell samples should be stored in extreme cold or -80” C in a freezer for at least 5 to 24 hours before transferring them to the storage vessels.
  5. Thawing - When required, the cells can be carefully thawed using specialized techniques. Controlled thawing will prevent the manger to the cells and maintain their viability.

Build a secure future for your Little One!

As a responsible individual, your goal for your family’s future is to provide them with every feasible means to live a happy, healthy life for many years to come. You can take advantage of accessing bio-genetic building blocks in times of need. At Cryoviva Life Sciences, we have cutting-edge technologies and follow standardized care for stem cell banking, with the ultimate goal of restoring their health and vitality in case of any future medical emergencies.
Location: India

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